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KMID : 0614620030420010012
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Korean Journal of Gastroenterology
2003 Volume.42 No. 1 p.12 ~ p.19
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Effect of Helicobacter pylori on Gastric Epithelial Cell Kinetics and Expression of Apoptosis-related Proteins in Gastric Carcinogenesis
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Lee Kang-Moon
Lee Dong-Soo
Yang Jin-Mo
Ahn Byung-Min
Lee Eun-Hee
Yoo Jin-Young
Kim You-Joung
Chung In-Sik
Sun Hee-Sik
Park Doo-Ho
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Abstract
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Background/Aims: We investigated apoptosis, cell proliferation and the expression of apoptosis-related proteins in the gastric epithelial cells of Helicobacter pylori (H. pylori)-negative and positive patients.
Methods: TUNEL staining, immunohistochemical staining for Ki-67, p53, Bcl-2, Bcl-XL and Bax were performed on paraffin-embedded specimens obtained from 11 H. pylori-negative controls, 20 H. pylori-positive chronic gastritis, 10 chronic gastritis with intestinal metaplasia (IM), 11 adenoma, and 7 adenocarcinoma patients. Apoptosis and proliferation were expressed as apoptosis index (AI) and proliferation index (PI).
Results: In H. pylori-positive groups, apoptosis and proliferation were increased compared with controls. However, an AI/PI ratio was highest in chronic gastritis cases and then gradually reduced according to the progress from IM to cancer. p53 was expressed in 28.6% of adenocarcinoma cases. Expression of Bax was significantly increased in chronic gastritis and IM patients compared with controls.
Conclusions: H. pylori infection induces marked increase in apoptosis and proliferation of gastric mucosa. In the premalignant lesions, the balance between apoptosis and proliferation is altered, and this imbalance may play a key role in the gastric carcinogenesis.
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KEYWORD
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Helicobacter pylori, Apoptosis, Gastric carcinogenesis, Protein p53, Bax
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